The Carbohydrate Moiety of Bovine Pancreatic Deoxyribonuclease
نویسندگان
چکیده
منابع مشابه
The carbohydrate moiety of bovine pancreatic deoxyribonuclease.
Preceding experiments by Price and his associates establish that bovine pancreatic deoxyribonuclease is a glycoprotein which contains glucosamine and mannose. In order to determine the mode of attachment of the carbohydrate to the enzyme molecule, chromatographically purified deoxyribonuclease has been digested with Pronase. Gel filtration of the digest yielded a fraction containing all of the ...
متن کاملBovine pancreatic deoxyribonuclease D.
Of the four deoxyribonucleases, DNases A, B, C, and D, observable by chromatography of preparations of the pancreatic enzyme on cellulose phosphate, DNase D, the component present in the smallest amount, has not been previously characterized. Peptide maps show that the sequence of the amino acid residues in the polypeptide chain of DNase D is indistinguishable from that of DNase C; analysis of ...
متن کاملProperties of chromatographically purified bovine pancreatic deoxyribonuclease.
Preparations of bovine pancreatic deoxyribonuclease obtained by the ammonium sulfate precipitation procedure of Kunitz can be further purified by chromatography on sulfoethyl-Sephadex at pH 4.70. The instability of earlier preparations of the enzyme has usually been attributed to the sensitivity of the protein to proteolytic digestion. This hypothesis has been confirmed; treatment of the starti...
متن کاملComparison of the multiple forms of bovine pancreatic deoxyribonuclease.
The earlier resolution of preparations of bovine pancreatic deoxyribonuclease into two active fractions by chromatography on sulfoethyl-Sephadex has been improved through the use of columns of phosphocellulose. Gradient elution with sodiwn acetate at pH 4.7 shows the presence of at least four forms of the enzyme. The principal component (DNase A) is accompanied by Fractions B and C, and, in ver...
متن کاملPutative secondary active site of bovine pancreatic deoxyribonuclease I.
Previous structural studies based on the co-crystal of a complex between bovine pancreatic deoxyribonuclease I (bpDNase I) and a double-stranded DNA octamer d(GCGATCGC)(2) have suggested the presence of a putative secondary active site near Ser43. In our present study, several crucial amino acid residues postulated in this putative secondary active site, including Thr14, Ser43, and His44 were s...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1969
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)91876-4